Bacterial Isolated from <em>Oreochromis niloticus</em> and Possible Use as Probiotic

• DOI: 10.20944/preprints202309.0142.v1
• Publication Date: 2023-09-05T01:44:50Z
• Authors: Luciana Yuri Sato; Danielle de Carla...; Mariene Miyoko Na...; Miguel Frederico ...; Maria José Ranzan...; Luara Lucena Cass...; Carlos Massatoshi...; Mateus Cardoso Gu...; Miguel Angel Mori...; Silvana Tapia-Pan...; Erna Elisabeth Bach; Ricardo Harakava; Leonardo Tachibana
• Keywords: Nile tilapia; Bacillus pumilus; Enterococcus faecium

The aim of this paper was to test isolated and pre-selected bacteria strains from Nile tilapia intestinal mucus, collected in Brazilian commercial fish farming, use as probiotics the feeding. variable were growth performance, hematology, microbiology immunology. These submitted vitro inhibition tests pathogenic bacteria. Then, they identified by PCR sequencing, comparing using BLAST software. experiment carried out Fisheries Institute, Sao Paulo, Brazil, for 84 days, following randomized design with seven treatments three replicates (21 experimental units): T0 &amp;ndash; control, T1 Bacillus velezensis, T2 pumilus, T3 subtilis, T4 &amp;ndash;Enterococcus hirae T5 Enterococcus faecium T6 mix used experiment. variables analyzed were: DGGE (Denaturing gradient gel electrophoresis), phagocytes respiratory burst activity, hematology bromatology. As result, B. pumilus (T2) all (T6), included diet, promoted better biomass values, similar feed conversion rate control group (p&amp;lt;0,05). Apparently, microorganisms does not affect health well-fare fish. index, except basophils thrombocytes numbers do demonstrated differences among groups, well glycemia, lactate level, lysozyme activities. In conclusion, mucus tilapia, (T6 - E. faecium) improve performance may be tilapia.