Optimization of Fungicidal and Acaricidal Metabolite Production by Aspergillus sp. SPH2

• DOI: 10.20944/preprints202311.1175.v1
• Publication Date: 2023-11-20T01:55:17Z
• Authors: Nicolas Reyes Cas...; Carmen E. Díaz; Maria Fe Andres; Juan Imperial; Félix Varcacel; Azucena González ...
• Keywords: Secondary metabolite; Liquid culture

The endophytic fungus Aspergillus sp. SPH2 was isolated from the stems of endemic plant Bethencourtia palmensis and its extracts were found to have strong fungicidal effects against Botrytis cinerea ixodicidal Hyalomma lusitanicum at different fermentation times. In this study, grown using three culture media two methodologies, Microparticulate Enhancement Cultivation (MPEC) Semi-Solid Fermentation (SSF), increase production secondary metabolites in submerged fermentation. addition an inert support medium (SSF) resulted a significant extract production. However, when talcum powder added media, unexpected results observed, with decrease biocompounds interest. Metabolomic analyses showed that aspergillic, neoaspergillic, neohydroxyaspergillic acids peaked first few days fermentation, notable differences observed among methodologies media. Mellein particularly affected by medium. These highlight importance surface properties morphology spores mycelia during fungal species.