Assessing the <em>In Vitro</em> Cytotoxicity, Antitumoral and Antioxidant Properties of Polyphenol‐rich <em>Perilla leaves </em>Extracts

• DOI: 10.20944/preprints202311.1599.v1
• Publication Date: 2023-11-27T02:02:17Z
• Authors: Gigi Adam; Florina Daniela C...; Liliana Verestiuc; Oana Cioanca; Ingrid Andrada Va...; Ana-Maria Adam; Cornelia Mircea; Aurel Nechita; Valeriu Romulus H...; Bogdan Huzum; AnaMaria Harabor; Monica Hancianu

(1) Background: This study aimed to outline the antitumoral, antioxidant, and cytotoxic proprie-ties of various types of Perilla frutescens extracts obtained from the leaves of the species; (2) Methods: We determined total polyphenols, flavonoids and anthocyanins contents, as well as the in vitro antioxidant, antitumoral, and cytotoxic actions in 3 types of ethanolic extracts (E1, E2, E3) and in 3 types of ethanol: acetone extracts (A1, A2, A3) of Perilla frutescens according to standardized procedures; (3) Results: We found that Perilla frutescens ethanolic extracts had higher total phenol concentrations (p= 0.002). Flavonoids and anthocyanins concentrations were not statistically different between the two extracts. The iron chelating capacity, hydroxyl radical scavenging capacity, superoxide anion radical scavenging capacity, and lipoxygenase inhibition capacity showed a significant increase with higher concentrations of Perilla frutescens extracts, particularly the ethanolic extracts. Perillyl alcohol had greater cytotoxic capacity on MG-63 cell line and E1 extract showed similar significant cytotoxic effects on A431 cell line; (4) Conclusions: Both ethanolic and ethanol: acetone extracts from Perilla frutescens exhibited important antioxi-dant and antitumoral actions in vitro, which proportionally increased with concentration. The cytotoxic threshold determined in this study for various types of extracts could help determine the best dosage with the maximum antioxidant and antitumoral potential.