Role of RPA Phosphorylation in the ATR-dependent G2/M Checkpoint and DSB Repair

• DOI: 10.20944/preprints202311.1616.v1
• Publication Date: 2023-11-27T02:02:17Z
• Authors: Shengqin Liu; Brendan M Byrne; Thomas N Byrne; Gregory G Oakley
• Keywords: CHEK1; Replication protein A

Cells respond to DNA double-strand breaks by initiating DSB repair and ensuing a cell cycle checkpoint. The primary responder is non-homologous end joining, an error prone pathway. However, when DSBs are generated after replication in the G2 phase of cycle, second pathway, homologous recombination, can come into action. Both ATM ATR important for DSB-induced checkpoint responses. One method working together through end-resection DSBs. As readout marker end-resection, RPA phosphorylated at Ser4/Ser8 N-terminus RPA32 response Here, significance phosphorylation damage, specifically S examined. synchronized cells necessary increases TopBP1 Rad9 accumulation on chromatin full activation ATR-dependent In addition, our data suggest modulates ATM-dependent KAP-1 Rad51 loading cells.