Antimicrobial and Antibiofilm Effects of Enterocins Against Methicillin-Resistant <em>Staphylococcus aureus</em> Strains From Rabbits

• DOI: 10.20944/preprints202311.1974.v1
• Publication Date: 2023-12-04T00:49:36Z
• Authors: Monika Pogány Sim...; Natália Zábolyová; Andrea Lauková

There is a major problem with the rising occurrence of highly virulent and multiply-resistant strains, including methicillin-resistant Staphylococcus aureus (MRSA), because their difficult treatment. This study aimed to evaluate antibacterial antibiofilm effect new enterocins (Ent) against potential pathogenic MRSA strains isolated from rabbits. Staphylococci (n =110) were identified PCR screened for methicillin/oxacillin/cefoxitin resistance (MR) using disk diffusion method PBP2&amp;#039; Latex Agglutination Test Kit. Enzyme production, hemolysis, DNase activity, slime biofilm formation tested in strains. The susceptibility eight partially-purified produced by E. faecium durans was checked agar spot tests. activity Ents quantitative plate assay. Out 14 MRSA, PBP testing confirmed MR 8 majority showed β-hemolysis. Slime production moderate observed all susceptible (100–12800 AU/mL; except Ent4231). (except Ent4231) noted high range (64.9-97.0%). These results indicate that offer promising option prevention treatment bacterial infections caused biofilm-forming MRSA.