Unveiling the Probiotic Potential of Streptococcus thermophilus MCC0200: Insights from In-vitro Studies corroborated with Genome Analysis

• DOI: 10.20944/preprints202312.0535.v1
• Publication Date: 2023-12-08T05:10:55Z
• Authors: Neelam Kapse; Vaidehi Pisu; Tanisha Dhakephalkar; Prajakta Margale; Deepa Shetty; Shilpa Wagh; Sumit Dagar; Prashant Dhakepha...
• Keywords: adhesion; <i>Streptococcus thermophilus</i>; health-promoting; QH301-705.5; anti-hypercholesterolemic activity; GRAS; gastrointestinal transit; Biology (General); Article

Streptococcus thermophilus is widely used as a starter culture in the dairy industry and has garnered attention beneficial bacterium owing to its health-promoting functionalities humans. In this study, probiotic potential of S. MCC0200, isolated from product, was investigated through combinatorial approach in-vitro in-silico studies. MCC0200 demonstrated ability survive harsh gastro-intestinal transit (GIT), adhere intestinal mucosa exert traits These findings were corroborated with evidence, wherein genome harboured genes associated tolerance GIT conditions, adhesion colonization. Genome mapping also highlighted produce compounds advantageous for host (folate, bacteriocins), release enzymes that can reinforce (antioxidative enzymes), metabolize food components be harmful sensitive people (lactose). positive effect on reducing cholesterol levels, proving candidate pharmaceutical applications. Absence transmissible antibiotic resistance virulence underscored GRAS nature MCC0200. This study explored probable applications beyond industry.