Comparative Genomic Analysis of <em>Colletotrichum lini </em>Strains with Different Virulence on Flax

• DOI: 10.20944/preprints202312.0929.v1
• Publication Date: 2023-12-15T01:19:27Z
• Authors: Ekaterina M. Dvor...; Elizaveta A. Sigova; Timur D. Mollaev; Tatiana A. Rozhmina; Ludmila P. Kudrya...; Roman O. Novakovskiy; Anastasia A. Turba; Daiana A. Zhernova; Elena V. Borkhert; Elena N. Pushkova; Nataliya V. Melni...; Alexey A. Dmitriev
• Keywords: Retrotransposon

Colletotrichum lini is a flax fungal pathogen, comprising differently virulent strains. In this work, we sequenced the genomes of three C. strains different virulence: high #390-1, medium #757, and low #771. We obtained more than 100× genome coverage with Oxford Nanopore Technologies reads 50× Illumina data. To construct high-quality genomes, several assembly strategies were tested. The final assemblies using Canu – Racon ×2 Medaka Polca scheme 54.0-55.3 Mb in length, contained 26-32 contigs, had an N50 &amp;gt; 5 BUSCO completeness 96%. Comparative analysis constructed showed similarity between mitochondrial nuclear genomes. However, rearrangement event loss 0.9 contig revealed. candidate effectors predicted sets proteins received after annotation. sequences extend knowledge on genetic diversity species are basis for establishing molecular mechanisms pathogenicity pathogen effective control anthracnose.