TLR Agonists Modify NK Cell Activation and Increase Its Cytotoxicity in Acute Lymphoblastic Leukemia

• DOI: 10.20944/preprints202404.1527.v1
• Publication Date: 2024-04-24T09:55:51Z
• Authors: Janet Gallardo-Za...; Erandi Pérez-Figu...; Víctor Olivar-López; Aurora Medina-Sanson; Elva Jiménez-Hern...; Enrique Ortega; Carmen Maldonado-...
• Keywords: NKG2D; Granzyme; Imiquimod; Janus kinase 3

Natural killer (NK) cells play a crucial role in innate immunity, particularly combating infections and tumors. However, hematological cancers, NK often exhibit impaired functions. Therefore, it is very important to activate its endosomal toll-like receptors (TLRs) as potential strategy restore antitumor activity. We stimulate from the peripheral blood mononuclear children with acute lymphoblastic leukemia isolated, were stimulated specific TLR ligands (Poly I:C, Imiquimod, R848, ODN2006) evaluated changes IFN-γ, CD107a, NKG2D, NKp44 expression, Granzyme B secretion, cytokine/chemokine release, cytotoxic Results revealed that Poly I:C Imiquimod enhanced activation of both immunoregulatory cells, increasing expression. R848 activated while ODN2006 boosted NKp44, IFN-γ secretion cells. also increased various cytokines/chemokines. Importantly, ODN 2006 significantly improved cytotoxicity against leukemic Overall, stimulation enhances cell activation, suggesting TLR8 (R848) TLR9 (ODN 2006) promising candidates for immunotherapy.