A Prebiotic Diet Containing Galactooligosaccharides and Polydextrose <span aria-describedby="tippy-24">Produces Dynamic and Reproducible Changes in the Gut Microbial Ecosystem in Male Rats

• DOI: 10.20944/preprints202405.0682.v1
• Publication Date: 2024-05-13T02:39:35Z
• Authors: Robert S Thompson; Samuel J Bowers; Fernando Vargas; Shelby Hopkins; Tel Kelley; Antonio Gonzalez; Christopher A Lowry; Pieter C Dorrestein; Martha Hotz Vitat...; Fred Turek; Rob Knight; Kenneth P Wright Jr.; Monika Fleshner
• Keywords: Prebiotic; Polydextrose; Metabolome

Despite substantial evidence supporting the efficacy of prebiotics for promoting host health and stress resilience, few experiments present documenting dynamic changes in microbial ecology fecal microbial-modified metabolites across time. Furthermore, literature reports a lack reproducible effects on specific bacteria bacterial-modified metabolites. The current examined whether consumption diets enriched (galactooligosaccharides, GOS polydextrose, PDX) compared to control diet, would consistently impact gut microbiome bile acids time between two research sites. Male Sprague Dawley rats were fed or prebiotic several weeks, their metabolome using 16S rRNA gene sequencing untargeted LC-MS/MS analysis. Dietary altered beta diversity, relative abundances bacterial genera, microbially modified PICRUSt2 analyses identified four inferred functional metabolic pathways by diet. Correlational network revealed deoxycholic acid as potential hub. All these reported consistent sites conclusion that dietary robustly changed ecosystem. Consistent with our previous work demonstrating GOS/PDX reduces negative impacts stressor exposure, we propose ingesting diet facilitates development health-promoting