Anticancer Potential of Valencia Peanut (<em>Arachis hypogaea </em>L.) Skin Extract against Cervical Cancer Cells In Vitro and in Nude Mouse Xenograft Models

• DOI: 10.20944/preprints202406.0761.v1
• Publication Date: 2024-06-13T06:58:10Z
• Authors: Jarckrit Jeeunngoi; Gulsiri Senawong; Sanun Jogloy; Jeerati Prompipak; Arunta Samankul; Suppawit Utaiwat; Khanutsanan Woranam; Banchob Sripa; Thanaset Senawong
• Keywords: HeLa; MTT assay

Abstract: This study aimed at investigating the effects of Valencia KK4-type peanut skin ethanolic extract (KK4-PSE) in combination with cisplatin or 5-fluorouracil (5-FU) against HeLa cells vitro and xenograft models. MTT assay showed that KK4-PSE, cisplatin, 5-FU suppressed proliferation a dose time dependent manner. Drug interactions by Chou-Talalay method demonstrated KK4-PSE enhanced antiproliferative activity index (CI) values 0.49 (48 h) 0.60 (72 h), indicating synergistic effect, while combined exhibited an additive effect (CI = 1.02) antagonistic 24 48 h exposures 1.12 1.18, respectively). In nude mouse models, markedly reduced tumor weights compared control single agent treatments. The achieved greater growth inhibition than KK4-PSE-cisplatin combination. mitigated hepatotoxicity induced both mice. spleen hyaloserositis was significantly treatment KK4-PSE. These findings suggest potential to inhibit cervical cancer cell alleviating toxicities 5-FU.