Aberrantly Expressed tRNA-Val Fragments Can Distinguish Canine Hepatocellular Carcinoma from Canine Hepatocellular Adenoma

• DOI: 10.20944/preprints202407.0990.v1
• Publication Date: 2024-07-12T12:08:54Z
• Authors: Saki Hashimoto; MD Nazmul Hasan; Mohammad Arif; Nobuhiro Nozaki; Al Asmaul Husna; Yu Furusawa; Takeshi Sogawa; Kaori Takahashi; Tomohide Kuramoto; Aki Noguchi; Masashi Takahashi; Osamu Yamato; Md Mahfuzur Rahman; Naoki Miura
• Keywords: Hepatocellular adenoma; KEGG

Hepatocellular adenoma (HCA) and hepatocellular carcinoma (HCC) can be difficult to differentiate but must diagnosed correctly as treatment prognosis for these tumors differ markedly. Relevant diagnostic biomarkers are thus needed, those identified in dogs may have utility human medicine because of the similarities between canine HCA HCC. A tRNA-derived fragment (tRF), tRNA-Val, is a promising potential biomarker mammary gland has not previously been investigated hepatic tumors. Accordingly, we aimed elucidate tRNA-Val HCC using clinical samples (tumor tissue plasma extracellular vesicles [EVs]) tumor cell lines with qRT-PCR assays. We also relevant functions signaling pathways bioinformatic analyses (Gene Ontology Kyoto Encyclopedia Genes Genomes). was markedly downregulated versus normal liver tissue, similar trend shown EVs healthy controls. Based on areas under receiver operating characteristic curves (AUCs), significantly distinguished (AUC=1.00, P=0.001) from controls (AUC=0.950, P=0.01). Bioinformatics analysis revealed that primarily involved DNA repair, mRNA processing, splicing linked N-glycan ubiquitin-mediated proteasome pathways. This first report expression HCA, its possible suggest could novel distinguish HCA. study provides evidence greater understanding role played by development