The Role of Propionate-Induced Rearrangement of Membrane Proteins in the Formation of the Virulent Phenotype of Crohn’s Disease-Associated Adherent-Invasive <em>Escherichia coli</em>

• DOI: 10.20944/preprints202408.1083.v1
• Publication Date: 2024-08-19T00:43:15Z
• Authors: Olga Vladimir Pob...; Maria Alexey Galy...; Elena Vladimir Mi...; Sergey Igor Koval...; Igor Pavel Smirnov; Alena Vyacheslav Lee; Lyubov Yuriy Fila...; Kirill Vitaly Sik...; Oleg Mikhayl Pana...; Alexey Yuriy Gorb...
• Keywords: Strain (injury)

Adhesive-invasive E. coli have been suggested to be associated with the development of Crohn's disease (CD). It is assumed that they can provoke onset inflammatory process as a result invasion intestinal epithelial cells, and then, due survival inside macrophages, stimulate chronic inflammation granuloma. In previous reports, we shown passage СD isolate ZvL2 on minimal medium M9 supplemented sodium propionate (PA) carbon source stimulates, glucose - inhibits adherent-invasive properties ability survive in macrophages. This effect was reversible not observed for laboratory strain K12 MG1655. We were able compare isogenic AIEС two phenotypes virulent (ZvL2-PA) non-virulent (ZvL2-GLU). showed that, unlike ZvL2-GLU, ZvL2-PA activates production ROS cytokines when interacting neutrophils. The does cause similar effect. Differences neutrophil NADH-oxidase activation &amp;zeta; potential ZvL2-GLU are changes abundance membrane proteins. Using differential 2D electrophoresis LC-MS, identified proteins may virulence ZvL2.