Neohesperidin Mitigates High-Fat Diet Induced Colitis <i>In vivo</i> by Modulating Gut Microbiota and Enhancing SCFAs Synthesis

• DOI: 10.20944/preprints202412.0473.v1
• Publication Date: 2024-12-09T03:53:08Z
• Authors: Kun Lu; Sijie Shan; Yanling Zeng; Guliang Yang
• Keywords: Proinflammatory cytokine

Previous research has consistently shown that high-fat diet (HFD) consumption can lead to the development of colonic inflammation. Neohesperidin (NHP), a naturally occurring flavanone glycoside in citrus fruits, anti-inflammatory properties. However, efficacy and mechanism NHP countering prolonged HFD-induced inflammation remains unclear. In this study, rats on HFD were intragastric administered (i.g.) with for 12 consecutive weeks. Results indicated natural compound is effective reducing colorectal at doses 40-80 mg/kg body weight by i.g. administration, significant decreases markers such as TNF-&amp;alpha; IL-1&amp;beta; levels. It also improved intestinal mucosal tissue integrity reduced HFD-stimulated via JAK2/STAT3 pathway. Furthermore, microbiota sequencing results showed intervention significantly downregulated Firmicutes/Bacteroidetes ratio. This ratio closely related preventive role context glycolipid metabolism disorder. Compared fecal cultures from group, after 48 h vitro fermentation, those group had distinct composition notably higher concentrations SCFAs. Collectively, these observations suggest NHP's biological activities downregulating are achieved regulating flora promoting SCFAs formation.