Outbreak of Carbapenem Resistant High-Risk Clone ST244 <i>Pseudomonas aeruginosa</i> in Dogs and Cats in Algeria

• DOI: 10.20944/preprints202502.0600.v1
• Publication Date: 2025-02-12T01:23:41Z
• Authors: Amina Badis; Nouzha Heleili; Manel Merradi; Ammar Ayachi; Piera Anna Martino; Gabriele Meroni; Alessio Soggiu
• Keywords: clone (Java method)

Background/Objectives: Pseudomonas aeruginosa causes chronic infections in humans and animals, especially cats dogs. This bacterium's ability to adapt acquire antibiotic resistance traits may complicate exacerbate antibacterial therapy. study aimed evaluate the patterns, virulence factors form biofilm of P. strains isolated from Algerian dogs cats. Methods: Nineteen samples were collected healthy diseased Isolates studied for their patterns (disc diffusion method), formation (Microtiter assay) Whole genome sequenced (MinION). Results: (15 4 cats) isolated. Antibiotic phenotypes observed against amoxicillin-clavulanic acid (100%), meanwhile towards ticarcillin was 40% (dogs) 25% (cats), ticarcillin-clavulanic 13.33% respectively imipenem 75% (cats) 20% (dogs). 95% producers. Different antimicrobial genes (ARGs) found: beta-lactamase mainly PAO, OXA-494, OXA-50 OXA-396, aminoglycosides gene (aph(3&amp;rsquo;)-IIb), fosA fosfomycin catB7 phenicol. The main high risk STs ST244, 2788, 388 1247. A large panel detected: exoS, exoT, exoY, lasA, toxA, prpL, algD, rhIA others. Conclusions: genetic variety resistant virulent makes public health protection difficult. Continuous monitoring research compliance with One Health policy are needed solve this problem.