Cell-Permeable Microprotein from Panax Ginseng Protects Against Doxorubicin-Induced Oxidative Stress and Cardiotoxicity

• DOI: 10.20944/preprints202503.1675.v1
• Publication Date: 2025-03-26T05:23:44Z
• Authors: Bamaprasad Dutta; Shining Loo; Antony Kam; Xiaoliang Wang; Na Wei; Kathy Qian Luo; Chuan-Fa Liu; James P. Tam
• Keywords: Cardiotoxicity

(1) Background: Doxorubicin (DOX) is a frontline chemotherapeutic, but its side effects from oxidative stress leading to cardiotoxicity, pose significant challenges clinical use. We recently discovered novel family of proteolysis-resistant, cystine-dense, and cell-penetrating microproteins Panax ginseng that we term ginsentides. Ginsentides, such as the 31-residue TP1 coordinate multiple biological systems prevent vascular dysfunction endoplasmic reticulum induced by internal external stressors; (2) Methods: assessed protective ginsentide on DOX-induced cardiotoxicity using both in vitro functional studies H9c2 cardiomyocytes vivo animal models zebrafish ICR mouse models. In these models, examined stress, apoptosis, intracellular calcium levels, mitochondrial function, inflammatory responses, cardiac function; (3) Results: show protects against cytotoxicity mitochondria-rich reduces myocardial injury mice mitigating inflammation, calcium, dysfunction, well apoptosis-mediated cell death. Importantly, preserves cellular homeostasis without compromising anticancer potency DOX breast cancer cells; (4) Conclusions: Our findings highlight specific antioxidative function managing during treatment provide promising lead for developing cardioprotective peptides stress.