Modified Methylation Following Electrostimulation in a Standardized Setting - Complementing a Transcriptomic

• DOI: 10.20944/preprints202503.1987.v1
• Publication Date: 2025-03-28T00:52:39Z
• Authors: Biagio Di Pietro; Simona Villata; Anna Plaksienko; Tiziana Guarnieri; Simeone Dal Monego; Margherita Degasperi; Pietro Di Lena; Danilo Licastro; Claudia Angelini; Francesca Frascella; Lucia Napione; Christine Nardini

Electrical stimulation (ES) is widely employed in both clinical therapies and research settings where it has shown promise promoting tissue regeneration, wound healing also inflammation control. Research highlighted ES as a regulator of DNA demethylation, which plays critical role nerve regeneration cellular repair mechanisms. While the impact on epigenetic processes recognized, its broader effects functions, particularly healing, are less understood. We recently showed how impacts inflammatory states by modulating transcriptomic metabolomic profiles 3D vitro model human fibroblasts keratinocytes included collagen matrix, i.e even absence nervous system. Here we propose to deepen our exploration differential methylation, including investigation potential age acceleration with usage an clock. These results support 5V Direct Current stimuli observed earlier at transcriptional level influence processes, suggest involvement DC5 control senescent associated inflammation.