A preliminary study of concurrent gains and losses across gene expression profiles and comparative genomic hybridization in Taiwanese breast cancer patients

• DOI: 10.21037/960
• Publication Date: 2013-05-03
• Authors: Chi Cheng Huang; Jaan-Yeh Jeng; Shih‐Hsin Tu; Heng Hui Lien; Ching Shui Huang; Liang Chuan Lai; Eric Y. Chuang
• Keywords: Comparative genomic hybridization; Gene dosage; Copy number analysis; genomic DNA; Clinical Significance

Purpose: The aim of the study was to perform genome-wide characterization Taiwanese breast cancer at molecular level by integrating 2 microarray technologies: comparative genomic hybridization (CGH) for analyzing DNA copy number changes, and gene expression profiles determining transcriptional variations. Concurrent gains losses from same subject across contexts may indicate potential biomarkers endemic cancer. Methods: Fourteen pairs cancerous normal tissue were collected prospectively. Genomic DNA, messenger RNA, corresponding controls extracted fresh frozen samples. Affymetrix® U133 plus 2.0 Agilent® aCGH Human 105k microarrays used profiling detecting variation. declared if only significant changes in a coherent manner observed both array CGH platforms within subject. Results: Among 14 samples that assayed, 7 estrogen receptor (ER)-positive. most common repeated concurrent (in 33% samples) LAPTM4B, HRSP12, WISP1, SQLE, GINS4, LYZ , DSCC1 . For clinical ER status relevance, there 294 133 losses, these reduced 30 27 cytobands, respectively. more among ER-negative tumors 1p32, 1p34, 1q21-23, 17q25, whereas ER-positive tumors, gain 8p11 reported. 8p21 cancers. Conclusions: Breast oncogenesis could originate persist through transcription patterns. Genes with patterns chromosomal levels are likely serve as sporadic cancer. In current study, we identified several candidate genes specific cancer, their implications deserve extensive evaluation future.