Stromal cells promote chemoresistance of acute myeloid leukemia cells via activation of the IL-6/STAT3/OXPHOS axis

• DOI: 10.21037/atm-20-3191
• Publication Date: 2020-11-14T18:45:21Z
• Authors: Diyu Hou; Bin Wang; Ruolan You; Xiaoting Wang; Jingru Liu; Weiwu Zhan; Ping Chen; Tiandi Qin; Xuehao Zhang; Huifang Huang
• Keywords: 0301 basic medicine; 03 medical and health sciences; Original Article

Bone marrow stromal cells (BMSCs) are known to promote chemoresistance in acute myeloid leukemia (AML) cells. However, the molecular basis for BMSC-associated AML remains largely unexplored.The mitochondrial oxidative phosphorylation (OXPHOS) levels of were measured by a Seahorse XFe24 cell metabolic analyzer. The activity total or signal transducer and transcription activator 3 (STAT3) was explored flow cytometry Western blotting. Real-time quantitative PCR, blotting enzyme-linked immunosorbent assay (ELISA) used analyze expression interleukin 6 (IL-6) human BMSC line HS-5, IL-6 knocked out HS-5 CRISPR/Cas9 system.In this study, we observed that co-culturing with BMSCs heightened OXPHOS cells, thus promoting these cell-induced upregulation is dependent on activation STAT3, especially serine phosphorylated STAT3 (pS-STAT3) relationship among pS-STAT3, OXPHOS, chemosensitivity induced demonstrated inhibitor, which upregulated downregulated pS-STAT3 respectively. Intriguingly, remodeled secrete more IL-6, augmented stimulated their chemoresistance. knockout impaired ability activate increase cells.BMSCs promoted via IL-6/STAT3/OXPHOS pathway. These findings exhibit novel mechanism bone microenvironment from perspective.