Comparison of the effects of photon, proton and carbon-ion radiation on the ecto-calreticulin exposure in various tumor cell lines

• DOI: 10.21037/atm.2019.09.128
• Publication Date: 2019-10-31T04:03:37Z
• Authors: Yangle Huang; Yuanli Dong; Jingfang Zhao; Lijia Zhang; Lin Kong; Jiade Jay Lu
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

Accumulating evidence suggested that radiotherapy can activate anti-tumor immune responses by triggering immunogenic cell death (ICD) of tumor cells. Calreticulin is regarded as one the most important markers ICD. The surface translocation calreticulin (ecto-CRT) serves an "eat me" signal for phagocytosis dying cells, which plays a pivotal role in activating immunity. However, there limited knowledge describing effects proton and carbon-ion radiation on ecto-CRT exposure. Hence, we investigated exposure multiple human carcinoma lines irradiated comparison to photon.This study examined four cancer including A549 (lung adenocarcinoma), U251MG (glioma), Tca8113 (tongue squamous carcinoma), CNE-2 (nasopharyngeal carcinoma). Cell were with photon, or at 0, 2, 4, 10 Gy (physical dose). level was analyzed flow cytometry 12, 24, 48 h post-irradiation. median fluorescence intensity calculated FlowJo.All three types radial beam increased 4 time-dependent manner. Ecto-CRT significantly elevated 1.5-2.4 times over post-irradiation compared controls (P<0.05). Proton photon dose escalation. Photon (P<0.05), while rather than 2 Gy. When iso-physical post-irradiation, showed similar effectiveness photon. While has stronger increasing Gy, but changed oppositely (P<0.05).All increase equally effective inducing exposure, revealed different proton.