K. pneumoniae and M. smegmatis infect epithelial cells via different strategies

• DOI: 10.21037/jtd-23-493
• Publication Date: 2023-08-29T01:19:10Z
• Authors: Renjing Hu; Lin Wan; Xiaoyun Liu; Jie Lu; Xichi Hu; Xiaoli Zhang; Min Zhang

As the first line of defense, epithelial cells play a vital role in initiation and control both innate adaptive immunity, which participate development disease. Despite its therapeutic significance, little is understood about specific interaction between pathogenic microorganisms lung cells.In this study, we performed head-to-head comparison virulence infection mechanisms Klebsiella pneumoniae (K. pneumoniae) Mycobacterium smegmatis (M. smegmatis), represent Gram-negative/positive respiratory pathogens, respectively, cell models for time.Through scanning electron microscopy combined with bacterial experiments, confirmed ability K. M. strains to form biofilm cord factor out wall. has stronger adhesion intracellular retention ability, while more likely induce acute infection. These pathogens could stay proliferate stimulate secretion cytokines chemokines through gene transcription regulator. can promote crosstalk among other immune from very early stage by prompting pro-inflammatory cytokines. Meanwhile, there were significant correlations pneumonia higher levels interleukin-15 (IL-15), interleukin-1Rα (IL-1Rα), fibroblast growth (FGF) basic, granulocyte colony-stimulating (G-CSF). At same time, also led changes expression cytoskeletal proteins cells.Our results emphasized immunoprotection immunomodulation against exogenous microorganisms, indicating that different damaged host strategies induced varying responses. they provided important clues key factors dealing complicated pulmonary infections.