The synergistic effects of PM2.5 and high-fat diet on Th1/Th2 balance in model mice with asthma

• DOI: 10.21037/jtd-24-1139
• Publication Date: 2025-03-28T09:05:43Z
• Authors: Ruifeng Bai; Bingqian Liu; Tianshui Li; Heng Zhou; Xinyang Yue; Ying Liu; Yining Shan; Zhigang Li; Yongjie Wei; Jun Wu

Particulate matter, ambient particulate matter with an aerodynamic equivalent diameter ≤2.5 µm (PM2.5) is closely associated asthma, and a high-fat diet also risk factor for the condition. In many cities in China, exposure to PM2.5 consumption of coexist. The Th1/Th2 balance immunological foundation onset progression it more accurate describe asthma symptoms terms changes this balance. Therefore, aim study was investigate effects combined on immune. Given background, our examined diets proposed potential molecular mechanisms development induced by these factors. study, male BALB/c mice ovalbumin (OVA)-sensitized subjected either normal or were exposed filtered air one month. We evaluated using histopathology, enzyme-linked immunosorbent assays, transcriptome sequencing, quantitative polymerase chain reaction (PCR). found that increased secretion Th2-related inflammatory mediators, while Th1-related mediators. However, still predominantly favored Th2 skew. shifted toward Th2, whereas Th1. combination resulted less pronounced polarization compared alone. short-term both exacerbate but there opposite direction modulation