Mutational profile of colorectal cancer lung metastases and paired primary tumors by targeted next generation sequencing: implications on clinical outcome after surgery

• DOI: 10.21037/jtd.2018.10.72
• Publication Date: 2018-11-28T12:31:36Z
• Authors: Thomas Schweiger; Sandra Liebmann-R...; Olaf Glueck; Patrick Starlinger; Johannes Laengle; Peter Birner; Walter Klepetko; Dietmar Pils; Berthold Streubel; Konrad Hoetzenecker
• Keywords: 03 medical and health sciences; 0302 clinical medicine; 3. Good health

Pulmonary metastasectomy is one of the cornerstones in treatment oligometastatic colorectal cancer (CRC). However, selection patients who benefit from a surgical resection difficult. Mutational profiling has become an essential part diagnosis and malignant disease. Despite this, comprehensive data on mutational profile CRC its clinical impact context pulmonary sparse. We therefore aimed to provide complete status metastases (PM) corresponding primary tumors by targeted next-generation sequencing (tNGS), correlate with outcome variables.Case-matched, formalin-fixed paraffin embedded specimens lung (n=47) matched (n=24) were sequenced using TruSeq Amplicon Cancer Panel (Illumina platform). Penalized Cox regression models applied identify mutations prognostic impact.Mutations found most frequently APC, TP53 KRAS, both PM tumors. Concordance between was 83.5%. Adaptive elastic-net regularized identified being for time recurrence (EGFR, GNAQ, KIT, MET, PTPN11) overall survival (OS) (PDGFRA, SMARCB1, TP53).Our findings suggest that harbor variety conserved de novo mutations. could predicting after metastasectomy. Moreover, our rationale future therapies metastases.