Combination of the stromal vascular fraction and platelet-rich plasma accelerates the wound healing process: pre-clinical study in a Sprague-Dawley rat model

• DOI: 10.21037/sci.2019.06.08
• Publication Date: 2019-07-12T07:57:31Z
• Authors: Karina; Muhammad F. Samudra; Imam Rosadi; Irsyah Afini; Tias Widyastuti; Siti Sobariah; Melinda Remelia; Riris L. Puspitasari; Iis Rosliana; Tri I. Tunggadewi
• Keywords: Rat model

Background: Adipose-derived stem cells (ADSCs) improve wound healing owing to their angiogenic potency. However, the production of large quantities ADSCs for clinical applications is laborious. In this study, efficacy stromal vascular fraction (SVF; non-expanded ADSCs) combined with platelet-rich plasma (PRP) which contains abundant growth factors, was evaluated using an animal model. Methods: PRP from venous blood and SVF lipoaspirates were harvested six donors. PRP, SVF, + saline solution as a negative control injected second degree burn wounds in backs 24 male Sprague-Dawley rats. On seventh day after injection, rats euthanized analyzed microscopically macroscopically. Results: Wounds closed faster group than or alone groups, less inflammation, prominent signs re-epithelization, more skin appendages vessels, higher rate hair growth. No infection rat death occurred during trial. Conclusions: The combination may provide additive stimulatory effect support angiogenesis accelerate process; accordingly, potential alternative ADSC treatment.