PRC1 promotes cell proliferation and cell cycle progression by regulating p21/p27-pRB family molecules and FAK-paxillin pathway in non-small cell lung cancer

• DOI: 10.21037/tcr.2019.09.19
• Publication Date: 2019-09-30T12:54:03Z
• Authors: Zhigang Liang; Xinjian Li; Jian Chen; Haina Cai; Liqun Zhang; Chenwei Li; Jingjie Tong; Wentao Hu
• Keywords: Paxillin

This study aimed to demonstrate the function and molecular mechanism of protein regulator cytokinesis 1 (PRC1) in carcinogenesis non-small cell lung cancer (NSCLC).Bioinformatics analysis was performed. Cell culture plasmid construction were conducted for transfection. mRNA expression, proliferation, migration, cycle detected. Mice models also constructed. The relationship between PRC1 prognosis NSCLC patients analyzed.PRC1 expression higher tumor tissues than adjacent non-tumor (P<0.05). Cells transfected with high-expression (TOPO-PRC1 group) had stronger ability proliferation migration (P<0.05) along a lower incidence stay at G2/M phase low-expression plasmid. showed tumors obtained from mice TOPO-PRC1 group significantly grew faster, larger, heavier group. Among 150 patients, more likely have lymph node metastasis occur progress into an advanced stage (P<0.05), shorter survival Moreover, phosphorylation level, Cip1/p21 Kip1/p27 (P<0.01).PRC1 could promote progression through FAK-paxillin pathway molecules regulation level p21/p27-pRB family molecules. might be new promising therapeutic target NSCLC.