Perivascular Stem Cells Diminish Muscle Atrophy Following Massive Rotator Cuff Tears in a Small Animal Model

• DOI: 10.2106/jbjs.16.00645
• Publication Date: 2017-02-15T17:23:01Z
• Authors: Claire D. Eliasberg; Ayelet Dar; Andrew R. Jensen; Iain R. Murray; Winters R. Hardy; Tomasz J. Kowalski; Cameron A. Garagozlo; Kyle M. Natsuhara; Adam Z. Khan; Owen J. McBride; Peter I. Cha; Benjamin V. Kelley; Denis Evseenko; Brian T. Feeley; David R. McAllister; Bruno Péault; Frank A. Petrigliano
• Keywords: Muscle Atrophy; Tenotomy; Supraspinatus muscle

Rotator cuff tears are a common cause of shoulder pain and often necessitate operative repair. Muscle atrophy, fibrosis, fatty infiltration can develop after rotator tears, which may compromise surgical outcomes. This study investigated the regenerative potential 2 human adipose-derived progenitor cell lineages in murine model massive tears.Ninety immunodeficient mice were used (15 groups 6 mice). Mice assigned to 1 3 procedures: sham, supraspinatus infraspinatus tendon transection (TT), or TT denervation via suprascapular nerve (TT + DN). Perivascular stem cells (PSCs) harvested from lipoaspirate sorted using fluorescence-activated sorting into pericytes (CD146 CD34 CD45 CD31) adventitial CD31). received no injection, injection with saline solution, either at time index procedure ("prophylactic") weeks following surgery ("therapeutic"). Muscles procedure. Wet muscle weight, fiber cross-sectional area, analyzed.PSC treatment (prophylactic therapeutic injections) DN (therapeutic resulted less weight loss greater area than was demonstrated for controls (p < 0.05). The treated point postoperatively had fibrosis controls. There compared controls.Our findings significantly atrophy PSCs suggests that use have role prevention without leading increased infiltration.Improved quality setting increase success rates repair lead superior clinical