The Effect of Osteoclastic Activity on Tendon-to-Bone Healing

• DOI: 10.2106/jbjs.f.00409
• Publication Date: 2007-10-01T18:42:43Z
• Authors: Scott A. Rodeo; Sumito Kawamura; C. Benjamin Ma; Xiang-hua Deng; Patrick S. Sussman; Peyton Hays; Liang Ying
• Keywords: Male; Wound Healing; Anterior Cruciate Ligament Injuries; RANK Ligand; Osteoprotegerin; Osteoclasts; Tendons; 03 medical and health sciences; 0302 clinical medicine; Osseointegration; Animals; Rabbits; Anterior Cruciate Ligament

Healing of a tendon graft in bone tunnel depends on ingrowth into the interface between and bone. Excessive osteoclastic activity may contribute to resorption, widening, impaired healing. We hypothesized that inhibition by osteoprotegerin (OPG) would increase formation around anterior cruciate ligament reconstruction rabbit model, while increased due application receptor activator nuclear factor-kappa B ligand (RANKL) impair ingrowth.Sixty skeletally mature, male New Zealand White rabbits underwent bilateral reconstruction. OPG (100 microg per tunnel) or RANKL (10 was delivered tendon-bone with use synthetic calcium phosphate carrier vehicle. Twenty animals were killed at two, four, eight weeks after surgery. Two from each group prepared for histological evaluation, other used biomechanical testing.A significantly greater amount surrounded healing OPG-treated limbs compared controls RANKL-treated all time-points (p < 0.05). There fewer osteoclasts The average area smaller than = 0.003 two p 0.004 four weeks). femur-anterior ligament-tibia complex had stiffness 0.04).Osteoprotegerin improves grafted junction model.