Protocol for a Longitudinal Analysis of the Vaginal Microbiome from a Pregnant Cohort of African Women in Nigeria

• DOI: 10.21106/ijma.330
• Publication Date: 2020-02-18T16:28:09Z
• Authors: Nkechi Martina Od...; Chinedum A. C. On...; Oladapo O. Olayemi; Akinyinka O. Omig...
• Keywords: Vaginal flora

Background : The vaginal microbiota is an important component of the reproductive health women as it offers protection against urogenital infection. African are reported to have a colonized with high proportions strict anaerobes rather than lactobacillus - dominated microbes. These been associated pre-term birth and neonatal disease. prevalence pre- term (PTB) in Africa poses major challenge healthcare, hence clinical scientific attention focused on understanding causative mechanisms PTB. A pragmatic approach curbing PTB requires identification microbiome during various stages healthy pregnancy (the ‘normal’). This information will provide baseline data for future investigations that may cause ‘abnormal’). We present protocol longitudinal analysis cohort pregnant Southwest Nigeria.
 Methods propose recruit 51 Nigerian women, enrolling them into study at 17-21 gestational weeks. Two swab samples three milliliters blood would be collected enrollment. Sample collection repeated 27-31 weeks’ gestation, ?36 24-48 hours after 6 weeks post-partum. DNA extracted from 16S rRNA sequencing performed. Blood assayed by ELISA technique placental steroid hormones. Data statistically analyzed considered light microbial diversity, clinical, nutrition other data.
 Conclusion Global Health Implication Our set bring new insights apparently postpartum, which should serve investigation microbes useful prediction management preterm birth. It anticipated these facilitate personalized therapeutic consequently improve fitness Africa.
 Key words • Vaginal Pregnancy Pre-term Birth Nigeria Women Longitudinal Protocol
 
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