Flow-Injection Chemiluminescence Determination of Ofloxacin and Levofloxacin in Pharmaceutical Preparations and Biological Fluids

• DOI: 10.2116/analsci.22.1145
• Publication Date: 2006-08-09T05:34:49Z
• Authors: Hanwen Sun; Liqing Li; Xueyan Chen
• Keywords: Ofloxacin; Luminescence; Chemistry, Pharmaceutical; Anti-Infective Agents, Urinary; Cerium; Levofloxacin; Urinalysis; Sensitivity and Specificity; 01 natural sciences; Chemistry Techniques, Analytical; 0104 chemical sciences; Models, Chemical; Pharmaceutical Preparations; Flow Injection Analysis; Humans

A novel, rapid and sensitive analytical method is described for determination of ofloxacin and levofloxacin by enhanced chemiluminescence (CL) with flow-injection sampling. The method is based on the CL reaction of the Ce(IV)-Na2S2O4-ofloxacin/levofloxacin-H2SO2 system. The enhanced CL mechanism was developed and the optimum conditions for CL emission were investigated. The CL intensity was correlated linearly (r = 0.9988) with the concentration of ofloxacin (or levofloxacin) in the range of 1.0 x 10(-8) - 1.0 x 10(-7) g ml(-1) and 1.0 x 10(-7) - 6.0 x 10(-6) g ml(-1). The detection limit (S/N = 3) is 7 x 10(-9) g ml(-1). The relative standard derivation (RSD, n = 11) is 2.0% for ofloxacin at 4 x 10(-7) g ml(-1) and for levofloxacin at 6 x 10(-7) g ml(-1). This method has been successfully applied for the determination of ofloxacin and levofloxacin in pharmaceutical preparations and biological fluids with satisfactory results.