Determination of Ulifloxacin by Terbium-sensitized Fluorescence with Second-order Scattering and Its Applications

• DOI: 10.2116/analsci.23.1373
• Publication Date: 2007-12-11T05:48:37Z
• Authors: Xiaoli Wang; Bo Shen; Huichun Zhao; Linpei Jin
• Keywords: Ofloxacin; Luminescence; Quinolones; 01 natural sciences; Piperazines; Anti-Bacterial Agents; 0104 chemical sciences; Spectrometry, Fluorescence; Humans; Scattering, Radiation; Indicators and Reagents; Terbium; Fluoroquinolones; Norfloxacin

This paper reports the determination of ulifloxacin (UFX) by terbium-sensitized fluorescence using a second-order scattering method. UFX and Tb(III) ion form a fluorescence complex in aqueous solution, and its maximum excitation and emission wavelengths are located at 273 and 545 nm, respectively. In optimum conditions, the relative intensity at 545 nm has a linear relationship to the concentration of UFX in the range of 2.0 x 10(-8) - 1.0 x 10(-5) mol L(-1) and the detection limit is 3.9 x 10(-9) mol L(-1). The proposed method was applied to the determination of UFX in spiked human serum and urine satisfactorily. The luminescence property of UFX is also discussed by comparing with norfloxacin (NFLX) and ofloxacin (OFLX).