Ketamine affects the integration of developmentally generated granule neurons in the adult stage

• DOI: 10.21203/rs.2.10461/v4
• Publication Date: 2019-12-18T21:26:55Z
• Authors: Zhanqiang Zhao; Bing Li; Yuqing Wu; Xujun Chen; Yan Guo; Yang Shen; he huang
• Keywords: NeuN; Granule cell; Neurotoxicity

Abstract Background Ketamine has been reported to cause neonatal neurotoxicity in a variety of developing animal models. Various studies have conducted study the mechanism for general anesthetic use during period. Previous experiments suggested that developmentally generated granule neurons hippocampus dentate gyrus (DG) supported hippocampus-dependent memory. Therefore, this aimed investigate whether ketamine affects functional integration DG. For purpose，the postnatal day 7 (PND-7) Sprague-Dawley (SD) rats were divided into control group and (rats who received 4 injections 40 mg/kg at 1 h intervals). To label dividing cells, BrdU was administered three consecutive days after exposure; NeuN+/BrdU+ cells observed by using immunofluorescence. evaluate support memory, spatial reference memory tested Morris Water Maze 3 months old, which immunofluorescence used detect c-Fos expression cells. The caspase-3 measured Western blot apoptosis hippocampal DG.ResultsThe present results showed exposure did not influence survival rate 2 but interfered with these DG neural circuits caused deficit hippocampal-dependent tasks.ConclusionsIn summary, findings may promote more brain.