Characterization and evolutionary analyses of Pestivirus A isolated from cattle in China

• DOI: 10.21203/rs.2.12445/v1
• Publication Date: 2019-08-06T20:20:56Z
• Authors: Cun Liu; Fabiola Villanova; Élcio Leal; Lin Liang; Yanming Zhang; Shangjin Cui
• Keywords: Pestivirus

Abstract Background Bovine viral diarrhea virus (BVDV) is a single stranded RNA with worldwide distribution that belongs to the genus Pestivirus, family Flaviviridae. Infection of cattle BVDV causes range clinical syndromes and can result in major economic losses. Here, we reported two isolates obtained from serum collected Shandong province, China commercial fetal bovine serum. Results The isolates, named BJ-2013 BJ-2016, were both members Pestivirus A, belonged genotype 1a 1b, respectively. was an cytopathogenic biotype virus, meanwhile BJ-2016 non-cytopathogenic virus. 45.3% GC content, 12,305 nt (BVDV BJ-2013) and12,273 BJ-2016) length. Phylogenetic analyses showed 1B Pestvirus A closely related South Korea (JQ418634) US (JN380088). Simultaneously, 1A USA (AF091605) Germany (AF041040). Recombination analysis isolate (KJ541471) mosaic sequences derived 1A. Evolutionary suggested had similar relative rates dN/dS ratios. Comparison amino acid sequence indicated there 12 acids (EGNWLVNADRLM) insertion NS2 BJ-2013. Conclusion: In present study, isolated identified. evolutionary strains different regions close relationships. It also reminded us again risk trans-regional transmission contamination what must not neglect.