Pathogenic fungi-induced susceptibility is mitigated by mutual Lactobacillus plantarum in the Drosophila melanogaster model

• DOI: 10.21203/rs.2.12906/v3
• Publication Date: 2019-11-27T16:54:45Z
• Authors: Wanzhen Su; Jialin Liu; Peng Bai; Baocang Ma; Wei Liu
• Keywords: Pathogenic bacteria; Pathogenic fungus; Commensalism

Abstract Background Since animals frequently encounter a variety of harmful fungi in nature, their ability to develop sophisticated anti-fungal strategies allows them flourish across the globe. Extensive studies have highlighted significant involvement indigenous microbial communities human health. However, daunting diversity mammalian microbiota and host genetic complexity are major obstacles our understanding these intricate links between components, immune genotype, disease phenotype. In this study, we sought bacterium-fungus-Drosophilamodel systematically evaluate effects commensal bacteria. Results We isolated pathogenic fungal strain, Diaporthe FY, which was detrimental survival development Drosophila upon infection. Using Drosophilaas model system, Drosophila-associated Lactobacillus plantarumfunctioned as probiotic, protected flies from mortality induced by FY. Our results show that L. plantarumhindered growth FYin vitro, decreased rate FY-infected vivo, consequently mitigating toxicity FYto hosts. Additionally, presence plantarumoverrode avoidance oviposition on FY-associated substrates. Conclusions FYwas identified potential Drosophilapathogen. Commensal plantarummitigated susceptibility Drosophilato fungi, providing insight into natural interplay contribute animal health pathogenesis.