Diagnostic accuracy of the LAMP assay for Neisseria meningitidis

• DOI: 10.21203/rs.2.13073/v2
• Publication Date: 2020-05-19T00:41:59Z
• Authors: Shu- Jin Fan; Xu-Guang Guo; Hong-kun Tan; Yu-cheng Xu; Yuan-zhi Chen; Tian-ao Xie; Zhi-yong Pan; Yang Shi-ou; Li Qin; Li Xiao-Yan; Li Zhen-Xing
• Keywords: Standard curve; Area under curve

Abstract Background Neisseria meningitidis is a major cause of bacterial meningitis, and these infections are associated with high mortality rate. Rapid reliable diagnosis meningitis critical in clinical practice. However, this disease often occurs economically depressed areas, so an inexpensive, easy to use, accurate technology needed. We performed meta-analysis assess the potential recently developed loop-mediated isothermal amplification (LAMP) assay for detection meningococcus. Methods Pubmed, Embase, Web Science were searched identify original studies that used LAMP detect After pooling data, sensitivity specificity calculated, summary receiver operating characteristic (SROC) curve was determined, area under SROC computed determine diagnostic accuracy. Publication bias assessed using Deek's funnel plot. Results examined 14 within 6 publications. The had (94%) (100%) meningococcus all studies. (0.980) indicated overall accuracy assay. There no evidence publication bias. Conclusion has comparable culture PCR meningococcus, but less expensive easier use. suggest adoption especially areas.