Transcriptome analysis provides insights into the non-methylated lignin synthesis in Paphiopedilum armeniacum seed

• DOI: 10.21203/rs.2.13152/v3
• Publication Date: 2020-06-11T18:40:55Z
• Authors: lin fang; Xin Xu; Ji Li; Feng Zheng; Mingzhi Li; Jingwei Yan; Yuan Li; Xinhua Zhang; Lin Li; Guohua Ma; Aying Zhang; Fubin Lv; Kunlin Wu; Songjun Zeng
• Keywords: Phenylpropanoid; Orchidaceae; KEGG; Germ plasm

Abstract Backgrounds: Paphiopedilum is an important genus of the orchid family Orchidaceae and has high horticultural value. The wild populations are under threat extinction because overcollection habitat destruction . Mature seeds most species difficult to germinate, which severely restricts their germplasm conservation commercial production factors inhibiting germination largely unknown. Results: In this study, large amounts non-methylated lignin accumulated during seed maturation armeniacum ( P. ), negatively correlates with rate. transcriptome profiles at different development stages were compared explore molecular clues for synthesis. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis showed that a number genes associated phenylpropanoid biosynthesis phenylalanine metabolism differentially expressed. Several key in biosynthetic pathway displayed expression patterns lignification process. PAL , 4CL HCT, CSE upregulation was C H accumulation. CCoAOMT F5H, COMT maintained low level or down-regulated inhibit conversion typical G S lignin. Quantitative real-time RT-PCR confirmed altered levels these vegetative tissues. Conclusions: This work demonstrated plasticity natural polymer assembly provided better understanding mechanism seed-specific