The Oral Microbiome and Salivary Proteins Influence Caries in Children aged 6 to 8 years

• DOI: 10.21203/rs.2.13636/v3
• Publication Date: 2020-06-23T14:02:08Z
• Authors: Wang Chen; Qian Jiang; GuoWei Yan; DeQin Yang
• Keywords: Dental plaque; Veillonella; Pyrosequencing; Oral Microbiome; Fusobacterium; Early childhood caries; Fusobacterium nucleatum

Abstract Background: Oral microbiome and salivary proteins play a critical role in the occurrence development of caries. In this study, we used metagenomic metaproteomic analyses to explore microbiological proteinic biomarkers investigate etiology caries 6-8 years old children. Our study aims offer better comprehension these factors relationship with caries, findings might facilitate risk assessment provide basis for future prevention strategies. Methods: Children six eight living rural isolated areas including 40 caries-active subjects caries-free were recruited. Supragingival plaque unstimulated saliva collected 16S rDNA pyrosequencing isobaric tags relative absolute quantitation (iTRAQ) technique coupled quantitative nano-flow liquid chromatography-tandem mass spectrometry(LC-MS/MS), respectively. Results: We found 6 phyla 13 genera predominant all samples, differences abundances can be observed. The Alpha diversity analysis demonstrated that richness bacterial communities similar between children groups; LEfSe detected community Dialister, Selenomonas, Actinomyces, Mogibacterium group (P<0.05) Capnocytophaga, Fusobacterium, Desulfuromonadales, Haemophilus, Porphyromonas group(P<0.05). core was defined as 18 results proteome identified 9135 unique peptides 1662 from 20 samples. Two hundred fifty-eight differentially expressed groups. Conclusions: microbial has little effect on but some bacteria different abundance could considered potential addition, host factor are