The mmu_circ_0000335 inhibits M1 microglial-induced hippocampal neuronal apoptosis via the miR-19b-3p/SOCS1 axis in a mouse epilepsy model

• DOI: 10.21203/rs.2.16568/v1
• Publication Date: 2019-10-31T04:47:54Z
• Authors: Yong Zhu; Qiong Li; Weiping Kuang; Jun Lu; Qin Wang; Shuaishuai Xia; Wei Peng; Liang Li; Ping Yang
• Keywords: 0301 basic medicine; 03 medical and health sciences

Abstract Background : Increasing evidence has demonstrated that circular RNAs (circRNAs) participate in epileptogenesis, but the expression profile and role of circRNAs epilepsy remain unknown. A circRNA microarray was performed to examine epilepsy-related circRNAs. Bioinformatics analyses, luciferin reporter experiments real-time quantitative PCR (Rt-qPCR) vitro were demonstrate mechanism circRNA-mediated gene regulation microglial phenotype under epileptic conditions. Then, further confirm effect on nerve damage hippocampus, a mouse model established by intraperitoneal injection lithium chloride pilocarpine. Results: The data indicated 364 differentially expressed comparing control tissues. In particular, mmu_circ_0000335 significantly downregulated mice which confirmed Rt-qPCR. Overexpression promoted BV2 cell transformation into M2 macrophage increasing CD206, Arg1, Ym1 IL-10 while decreasing M1 markers IL-1β, IL-6, TNF-α IFN-γ expressions triggered upregulation Suppressor Cytokine Signaling 1 (SOCS1) miR-19b-3p levels, as determined luciferase assay. vivo studies found overexpression decreased epilepsy-induced neural apoptosis hippocampus reducing inflammatory cytokine expression. Immunofluorescence detection showed had an anti-inflammatory effect. Conclusions: These results collectively involved progression functioning sponge enhance SOCS1 Thus, may be candidate therapeutic target for patients.