Submicromolar copper (II) ions stimulate transretinal signaling in the isolated retina from wild type but not fromCav2.3-deficient mice

• DOI: 10.21203/rs.2.16907/v3
• Publication Date: 2020-04-01T20:09:19Z
• Authors: Jan Niklas Lüke; Felix Neumaier; Serdar Alpdogan; Jürgen Hescheler; Toni Schneider; Walid Albanna; Isha Akhtar-Schäfer
• Keywords: Erg; Wild type

Abstract Background : So far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca 2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from sensitivity b-wave stimulation Ni , Zn and Cu . To further confirm interpretation these findings, we compared effects application chelation (using kainic acid, KA) on neural retina wild type v 2.3-deficient mice. Furthermore, immediately effect KA ERG modulation assessed. Methods Transretinal recorded as an superfused murine isolated wildtype mice Results In mice, stimulating 100 nM CuCl 2 is absent retinae but prominent 2.3-competent Application up 3 mM tricine does not affect both genotypes, most likely because chelating amino acids present nutrient solution. 27 µM significantly increased amplitude 2.3 (-|-) can explained endogenous KA-receptors described horizontal, OFF-bipolar, amacrine or ganglion cells, which could fully blocked study. Conclusion: dependent occurs competent supporting ideas derived previous work bovine that channels are shaping responses during light perception.