Effects of pulsed in-feed antimicrobials on chicken fecal resistome

• DOI: 10.21203/rs.2.18210/v1
• Publication Date: 2019-12-05T23:46:07Z
• Authors: Siyuan Chen; Jintao Yang; Jiaxiong Zeng; Jie Hao; Ruonan Zhao; Zhenling Zeng; Wenguang Xiong
• Keywords: Resistome; Florfenicol; Chlortetracycline

Abstract Background: Antimicrobial resistance has become a global problem that poses great threats to human health. Antimicrobials are widely used in broiler chicken production and consequently affect the fecal resistome. Here we metagenomic approach investigate effects of pulsed antimicrobial administration on Chickens received three 5-day-course alone/combined antimicrobials at therapeutic dose, including amoxicillin, chlortetracycline florfenicol. Chicken feces was collected T0 (before treatment), T5 (after first T10 second T15 third treatment) age 40 days ready for slaughter. Results: Tetracycline gene polymyxin only responded respectively. The tetracycline tetC significantly increased from group. multidrug acrB, acrF, mdtA, mdtK CRP occurred significant increase amoxicillin group (P<0.05). Klebsiella decreased florfenicol group, while Amoxicillin did not lead change Klebsiella. Escherichia always major host genes. ARG-harboring mainly due selection β-lactam, tetracycline, chloramphenicol genes they harbored. Conclusions: results indicated induced by shaped microbiome We provided comprehensive insight into antimicrobial-mediated alteration These findings will give suggests veterinarian combating production.