Distribution of Macrolide-Lincosamide-Streptogramin B antibiotics resistance genes in clinical isolates of Staphylococci

• DOI: 10.21203/rs.2.19686/v1
• Publication Date: 2019-12-27T16:49:31Z
• Authors: Dharma Nagarkoti; Krishna Prajapati; Ajay Narayan Sharma; Arrogya Gyawali; Sarita Manandhar
• Keywords: Lincosamides; Macrolide Antibiotics

Abstract Background Staphylococci are the most commonly isolated pathogen from clinical specimen. These isolates posing threat due to increasing trend of antimicrobial resistance particularly methicillin. Macrolide-lincosamide streptogramin B family antibiotics is used treat such infections. This study was aimed detect prevalence inducible clindamycin and observation erm msr genes among tertiary care hospital Nepal.Methods different specimen were identified antibiotic susceptibility profile determined following Kirby Bauer disc diffusion method. The double or D-zone test as outlined in CLSI document M100-S24 performed examine isolates. Multiplex PCR for detection gene using specific primers ermA , ermB, ermC, msrA msrB genes.Results Of 60 isolates, 39 (65%) Staphylococcus aureus ( S. ) 21 (35%) coagulase negative (CNS) with 25 (64%) 15 (71%) representing methicillin resistant CNS respectively. Constitutive MLS phenotype observed 24 (40%) 14 (23%) respectively by D test. prevalent ermC (37%) followed (12%), (10%) A (10%). None found possess gene.Conclusions detected more than aureus. presence constitutive well necessitates minimize treatment failure. characteristic varies settings, geographical locations, patients’ demography etc. result this may help elucidate predominant characteristics Nepal.