Evaluation of RNA Purification Methods by Using Different Blood Stabilization Tubes: Identification of Key Features for Epidemiological Studies

• DOI: 10.21203/rs.2.23086/v1
• Publication Date: 2020-02-10T20:47:30Z
• Authors: Jose Antonio Carr...; Raul de la Puente...; Purificación Cata...; Juan David Rejon ...; Laura Espín-Vallejo; Veronica Valdivie...; Rocio Aguilar-Que...
• Keywords: Blood Specimen Collection; Science (General); Liquid biopsy; QH301-705.5; RNA Stability; R; Reproducibility of Results; Biomarker; Q1-390; Research Note; Epidemiologic Studies; RNA quality; Pre-analytics; Medicine; Humans; RNA; Biology (General); Biobank

Abstract Objetive Peripheral Blood is the most promising source of RNA biomarkers for diagnostic and epidemiological studies, because presence disease prognostic information reflected in gene expression pattern. Quality used by a number different downstream applications, so selection appropriate stabilization purification method important. We have analyzed purified from 300 blood samples 25 donors processed two technicians using three methodologies with Tempus PaxGene tubes. Results The best quality sample results were obtained Spin Isolation Kit miRNA Kit, although larger amounts Kit. Lower Cq values observed genes that tested respectively. identify as robust methodology, whilst MagMax Stabilized Tubes showed instability. For biobanks, which process large cohort conduct methodology. study demonstrates robustness real-life procedures.