NCAM and attached polysialic acid affect behaviors of breast epithelial cells through differential signaling pathways

• DOI: 10.21203/rs.2.24259/v2
• Publication Date: 2020-04-08T14:42:36Z
• Authors: Lin Cao; Xin Wang; Juhong Yang; Jia Guo; Xiang Li; Xiaomin Yang; Zengqi Tan; Feng Guan
• Keywords: Polysialic acid

<title>Abstract</title> Background Neural cell adhesion molecule (NCAM), a common mammalian surface glycoprotein, is the major substrate of polysialic acid (polySia). Polysialylated NCAM occurs in many types cancer, but rarely normal adult tissues. Epithelial-mesenchymal transition (EMT) an important process contributing to tumor metastasis. The functional role hypersialylation EMT unclear. Method Expression and polysialylated breast cancer progression were evaluated by western blot (WB), immunohistochemistry, semi-quantitative PCR immunoprecipitation (IP). Overexpression NCAM-140 ST8SiaII performed assess liposome transfection. Cell proliferation ability was investigated with MTT assay. Transwell wound closure assay conducted evaluate migratory ability. Phagokinetic gold sol motility ability, respectively. Furthermore, WB IP used reveal activated signaling pathway. Results present study indicates that attached affect behaviors epithelial cells through differential pathways, based on following observations: (i) aberrantly regulated cells; (ii) expression upregulated undergoing EMT; (iii) overexpression induced (iv) promoted migration activation β-catenin/slug pathway; (v) modification polySia inhibited EGFR/STAT3 Conclusion This demonstrates facilitate different pathway behaviors. Switching between NCAM-mediated pathways appeared depend decoration.