Altered Diversity and Composition of Gut Microbiota in Wilson's disease

• DOI: 10.21203/rs.2.24572/v1
• Publication Date: 2020-02-26T16:05:36Z
• Authors: Xiangsheng Cai; Lin Deng; Xiaogui Ma; Yusheng Guo; Zhiting Feng; Minqi Liu; Yubin Guan; Yanting Huang; Jianxin Deng; Hongwei Li; Hong Sang; Fang Liu; Xiaorong Yang
• Keywords: Adult; Male; 0301 basic medicine; Bacteria; Science; Q; R; Middle Aged; Article; Gastrointestinal Microbiome; 3. Good health; 03 medical and health sciences; Hepatolenticular Degeneration; Medicine; Humans; Female

Abstract Background: Wilson’s disease (WD) is a rare autosomal recessive inherited disorder of chronic copper toxicosis with high mortality and disability. Recent evidence suggests a correlation between dysbiosis in the gut microbiome and metabolic disease. Therefore, the impact of intestinal microbiota polymorphism in WD need to be explore for seeking some microbiota benefit for WD patients. Methods: In this study, the 16S rRNA sequencing was performed on fecal samples from 14 patients with WD and were compared to the results from 16 healthy individuals. The diversity and composition of the gut microbiome in the WD group were significantly lower than those in healthy individuals. Results: The WD group presented unique richness of Gemellaceae , Pseudomonadaceae and Spirochaetaceae at family level in WD group, which were hardly detected in healthy controls The WD group had a markedly lower abundance of Acidobacteria , Firmicutes and Verrucomicrobia , and a higher abundance of Bacteroidet es, Proteobacteria , Cyanobacteria and Fusobacteria than that in healthy individuals. The Firmicutes to Bacteroidetes ratio in the WD group was significantly lower than that of healthy control. The functional profile of the gut microbiome from WD patients showed a lower abundance of bacterial groups involved in the pathways of transcription factors and ABC-type transporters, compared to healthy individuals. The dysbiosis of gut microbiota may be influenced by the host metabolic disorders of WD such as signaling pathway of ABC-type transporters and multiple metabolic modules. Conclusions: This study provides a new understanding of the pathogenesis of WD and new possible therapeutic targets.