Rapid and inexpensive method of studying dedifferentiation in rabbit articular chondrocytes

• DOI: 10.21203/rs.3.pex-1618/v1
• Publication Date: 2021-09-16T15:48:49Z
• Authors: Fahad Hassan Shah; Young Seok Eom; Byung Su Ko; Yohan Han; Song Ja Kim
• Keywords: Chondrogenesis; Matrix (chemical analysis)

Abstract Chondrocyte dedifferentiation is a cellular phenomenon in which chondrocyte loses its chondrogenic characteristics and ability to synthesize extracellular matrix. These conditions commonly reported different cartilage degenerative diseases. The methods used induce cell models of chondrocytes are often time consuming or require expensive reagents. Here our protocol, we describe the utility using 2-deoxy-D-glucose as dedifferentiating agent for rabbit studying process.