Tandem repeats structure of gel-forming mucin domains could be revealed by SMRT sequencing data

• DOI: 10.21203/rs.3.rs-112828/v1
• Publication Date: 2020-11-25T01:32:58Z
• Authors: Tiange Lang
• Keywords: Threonine; 0301 basic medicine; Technology; Nucleotides; Science; Q; R; Article; 3. Good health; 03 medical and health sciences; Asian People; Tandem Repeat Sequences; Medicine; Humans

Abstract Background.

Gel-forming mucin domains of mucin genes show great complexity with tandem repeats (TRs), thus make it difficult to study the sequences. Methods.

With the coming of single molecule real-time (SMRT) sequencing technologies, we manage to present sequence structure of mucin domains via SMRT long reads for MUC2, MUC5AC, MUC5B and MUC6. Results.

Our study shows that for different individuals, single nucleotide polymorphisms (SNPs) could be found in mucin domains of MUC2, MUC5AC, MUC5B and MUC6, while different number of tandem repeats could be found in mucin domains of MUC2 and MUC6. Conclusions.

This information will provided new insights on getting the sequence for Tandem Repeat parts which locate in coding region.