Validating Blackberry Seedling Pedigrees and Developing an Improved Multiplexed Microsatellite Fingerprinting Set

• DOI: 10.21273/jashs04474-18
• Publication Date: 2018-10-19T12:43:59Z
• Authors: Jason D. Zurn; Katie A. Carter; Melinda H. Yin; Margaret Worthington; John R. Clark; Chad E. Finn; Nahla Bassil
• Keywords: Germ plasm; DNA profiling; Pedigree chart; Identification; Outcrossing

Confirming parentage and clonal identity is an important aspect of breeding managing germplasm collections clonally propagated, outcrossing crops, like blackberry ( Rubus subgenus ). DNA fingerprinting sets are used to identify off-cross progeny confirm identity. Previously, a six-simple sequence repeat (6-SSR) set was developed for using small number samples. The usefulness the 6-SSR pedigree confirmation had not been evaluated. Therefore, it in this study validate 6 12 biparental populations from University Arkansas (UA) US Department Agriculture Agricultural Research Service (USDA-ARS), Horticultural Crops Unit (HCRU) programs, respectively. Twenty-seven 489 individuals these were identified as off-cross. sufficient confirmation; however, total 61 plants distributed across 28 genotypes could be distinguished each other. An 8-SSR with improved resolution subsequently evaluate 177 accessions USDA-ARS National Clonal Germplasm Repository, UA, HCRU programs. all samples expected have unique differing fingerprints two suspected identical fingerprints. Cluster analysis grouped eastern western programs based on geography descent. Future work will focus establishing database identification determining relationships between accessions.