Phospholipid and Triglyceride keep vortex-dispersed sulfur mustard analog as undissolved droplets which induce necrosis from nearby cells via declining mitochondrial membrane potential

• DOI: 10.2131/fts.6.359
• Publication Date: 2019-12-18T22:04:02Z
• Authors: Feng Ye; Guorong Dan; Qinya Zeng; Mingliang Chen; Yuanpeng Zhao; Jin Cheng; Yan Sai; Jiqing Zhao; Zhongmin Zou
• Keywords: Lipid droplet

SM (Sulfur mustard) is an oily, hydrophobic, and lipophilic chemical agent that damages cells with intricate patterns. CEES (2-chloroethyl ethyl sulfide soluble) a standard analog commonly employed in the toxicity mechanism study. To obtain ideal results vitro, researchers should disperse well medium, avoiding presence of undissolved droplets. However, such purpose not easy to reach under conventional solution preparation, information about droplet formation function little available. Here, we showed phospholipid triglyceride, two essential components serum lipids, could prevent from dissolving water after vortex, which kept partial as small By detecting level, proved residual droplets slowed hydrolysis conversion. Under microscope, were observed degrade diffuse time induce necrosis mitochondrial membrane potential decline nearby cells. In conclusion, damage pattern quite different dissolved CEES, low level triglyceride beneficial for preventing preparing solution.