PCR Assays for the Lr37‐Yr17‐Sr38 Cluster of Rust Resistance Genes and Their Use to Develop Isogenic Hard Red Spring Wheat Lines

• DOI: 10.2135/cropsci2003.1839
• Publication Date: 2010-07-29T19:48:25Z
• Authors: M. Helguera; I. A. Khan; J. Kolmer; D. Lijavetzky; L. Zhong‐qi; J. Dubcovsky
• Keywords: Sequence-tagged site; Introgression; Common wheat; Stem rust

Rust resistance genes Lr37 , Sr38 and Yr17 are located within a segment of Triticum ventricosum (Tausch) Cess. chromosome 2NS translocated to the short arm bread wheat 2AS. Characterization this by 13 restriction fragment length polymorphism (RFLP) markers indicated that translocation replaced approximately half 2A (distal 25–38 centimorgans, cM). The objective study was develop polymerase chain reaction (PCR) assays based on RFLP marker cMWG682 facilitate transfer cluster rust into commercial ( aestivum L.) cultivars. DNA sequence obtained from A‐, B‐, D‐, N‐alleles used design N‐allele specific primers. amplified PCR primers cosegregated with presence RFLP‐2NS band in all backcross populations. A cleaved polymorphic (CAPS) for 2A‐allele. This can be differentiate homozygous heterozygous plants carrying final cycle introgression or screenings segregating Finally, third assay developed means TaqMan technology as high‐throughput alternative selection 2NS/2AS large populations breeding programs have access real time equipment. These molecular were four hard red spring isogenic lines segment. One lines, derived ‘Anza,’ did not show expected spite Analysis F 1 hybrids suggested suppressor gene is present Anza. will provide valuable tool test effects quality agronomic performance.