Production of γ‐Linolenic Acid and Stearidonic Acid in Seeds of Marker‐Free Transgenic Soybean1

• DOI: 10.2135/cropsci2004.6460
• Publication Date: 2014-07-18T18:25:46Z
• Authors: Shirley Sato; Aiqiu Xing; Xingguo Ye; Bruce Schweiger; Anthony Kinney; George Graef; Tom Clemente
• Keywords: Selectable marker; Linolenic acid; gamma-Linolenic acid

Through a single desaturation step, the Borago officinalis L. Δ 6 desaturase can convert linoleic acid and α‐linolenic to γ‐linolenic (GLA) stearidonic (STA), respectively. Both GLA STA are of interest pharmaceutical nutraceutical industries. Production these fatty acids is costly. One potential strategy reduce production cost would be generate them in major oilseed crop. To this end, cDNA B. ‐desaturase gene was cloned downstream embryo‐specific promoter β‐conglycinin. The resultant cassette assembled into two T‐DNA binary vector, which second element harbored selectable marker cassette. final plasmid subsequently used transform soybean [ Glycine max (L.) Merr.]. simultaneous delivery elements as derive progeny transgenic for free T‐DNA. Twenty‐nine lines were recovered that both elements, 17 produced seed storage lipids. Average levels ranged from 3.4 up 28.7%, while varied just under 0.6 4.2% T 1 generation. Among STA, four identified element.