Validating the Fhb1 QTL for Fusarium Head Blight Resistance in Near‐Isogenic Wheat Lines Developed from Breeding Populations

• DOI: 10.2135/cropsci2006.03.0206
• Publication Date: 2007-01-23T01:58:53Z
• Authors: Michael O. Pumphrey; Rex Bernardo; James A. Anderson
• Keywords: 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences

ABSTRACT Quantitative trait loci (QTLs) have been identified for numerous species since the 1990s using populations developed from biparental crosses. The most common methods of validating QTLs are to quantify their effects in additional mapping or test near‐isogenic lines (NILs) original population. These approaches QTL validation fail adequately examine effectiveness a breeders' populations. We an alternative method which NILs existing breeding segregating QTL. Our objective was validate this Fhb1 , major Fusarium head blight [FHB; causal agent graminearum (Schwabe)] resistance wheat ( Triticum aestivum L.). Microsatellite markers flanking region were used develop 19 QTL‐NIL pairs by sampling F 3:4 families 13 different Each pair tested greenhouse point‐inoculation experiment and four field FHB screening nurseries. Near‐isogenic with allele had significant P < 0.001) average reductions 23% disease severity ratings 27% infected kernels harvested grain. Disease spread 9 out total significantly 0.05) lower experiments when present. methodology employed study should be broadly applicable other quantitative traits plant species.