Mode of Reproduction of Colchicine‐Induced Paspalum plicatulum Tetraploids

• DOI: 10.2135/cropsci2008.05.0270
• Publication Date: 2009-07-01T02:21:49Z
• Authors: María E. Sartor; Camilo L. Quarin; Francisco Espinoza
• Keywords: Apomixis; Paspalum; Sexual reproduction; Parthenogenesis; Bivalent (engine)

Paspalum plicatulum Michx. is a wild forage grass species. The common races are tetraploid and apomictic, while sexual diploid representatives have been reported sporadically. Objectives of this study were to induce 4 x individuals from diploids, determine the capacity for hybridization with other apomictic species closely related P. , thus create material cross compatible Plicatula group . Two induced plants recovered germinating 2 seeds treated 24 h colchicine. Bivalent quadrivalent chromosomes most association at meiosis in these autotetraploids. Embryological analysis progeny tests using molecular markers revealed that both tetraploids reproduced sexually. Single‐seed screening by flow cytometry confirmed full reproduction. These retained high self‐incompatibility system but set seed after reciprocal crosses when crossed pollen guenoarum Arechav. group. Both constitute foundational plant improvement through gene exchange selection possibly several